Once the Law is signed it goes into effect and violators are subject to prosecution.
No, there is no one fighting it and you can only appeal a conviction, not a law.
Understand this; The Online Gambling Prohibition section is only a small part of the overall “law” it is attached to which is the Port Security Act. No one is about to challenge a law designed to enhance the security of US Ports against terrorists. That would be political suicide. We are on our own this time. Thats exactly WHY Frist chose this sort of a bill to attach the prohibition act to. This devious bastard knew exactly what he was doing.